Ochratoxin A (OTA), a mycotoxin that contaminates food and feed products, is naturally produced by Aspergillus and Penicillium species. Nephrotoxicity, hepatotoxicity, neurotoxicity, teratogenicity, mutagenicity, and immunotoxicity are only a few of the serious health risks caused by OTA. In this investigation, a total of 69 food and feed samples were collected from retail market in Sharika governorate and used as a source of the isolated fungi. Two hundreds and ninety-two fungal strains were isolated, morphologically identified and their ability to produce OTA was tested. Among these fungi, only 60 strains, belonging to 3 fungal species, were able to produce OTA. Three fungal strains viz. A. fumigatus H11, A. terreus DF6, and A. niger T2, isolated from samples of hazelnuts, dried figs, and tomatoes, respectively; were the most potent ochratoxigenic strains among these 60 ochratoxigenic fungal strains. These three fungal strains were molecularly identified, and their sequences were successfully deposited in the Genebank with accession numbers; A. fumigatus MW411792.1, A. terreus MW512515.1 and A. niger MW513392.1.