This study was carried out to investigate the effect of endophytic fungi on physiological responses of Galleria mellonella (G. mellonella). The analyses of some parameters have been recorded as influenced by the filtrate of A. nidulans, A. flavus and A. niger. After 3 days, A. flavus AUMC 13942, A. niger AUMC 13944 strain produced the highest GOT activity of up to 2.16 and 1.29 µg pyruvate\ g. b. wt\ min, respectively. The increase in incubation period caused a notable decrease in GOT activity of G. mellonella larvae with the treated fungal strains. A. flavus AUMC 13942 causes a significant decrease (P<0.05) in GOT enzyme activity after 5 days then causes disturbance in its values to the end of experiment, these values are 1.25 and 0.13 µg/ g.b.wt after 7 and 9 days respectively. A. nidulans, A. niger and A. flavus cause the highest increase in the enzyme activity after 3 days and their values are 42.27, 21.91 and 8.81 µg/ g.b.wt respectively, then decrease again until the end of experiment. It can be seen that, the highest increase recorded after treatment of LC50 of A. nidulans after 3 days, while the lowest value of the enzyme recorded 0.11 after treatment with LC50 of A. niger after 9 days of treatment. Sub lethal concentrations of A. nidulans AUMC 13941 produce the highest increase in ACP enzyme activity at the 3rd day with value (106.43 µg/ g).