Background: Supporting nurses in health care sectors is very important, as nurses are the backbone of the hospital, perceived organizational supportive practices play an important role in improving nurses' work-life balance. Aim: to assess the relation between perceived organizational support and work-life balance among nurses. Subjects and Method: A descriptive correlational design was utilized in this study. Setting: The study was conducted at Al Mabara Hospital for Health Insurance - El Zagazig, Alsharaqia, Egypt. Subjects: Convenience sample (410) staff nurses. Tools: Data were collected using two tools: The perceived organizational support scale and the Work-life balance survey. Results: 97.6% of studied nursing staff had a low perception level of organizational support and 90.2% of them had a moderate level of work-life balance. Conclusion: There was a positive significant correlation between work-life balance and organizational support. Recommendations: Hospital management should create strategies that promote a supportive work environment such as rewards, flexible work schedules, fair pay and benefits, safe working conditions, and training should be made available for nurses to improve the balance between work and life which in turn will give satisfaction in their personal life.