Background: Attention Deficit Hyperactivity Disorder (ADHD) is a neurodevelopmental illness that often first appears in infancy and lasts well into adulthood. Mothers of children with ADHD often face unique challenges that might impact their mental health and the dynamics of their homes. The relation between these mothers' anxiety, depression, and family functioning has been the subject of research and therapeutic attention. Aim of the study: This study aimed to examine the relation between depression, anxiety, and family functioning among mothers of children with ADHD. Subjects and Methods: Research Design: This study used a descriptive approach to achieve its goal. Setting: This study was performed at pediatric outpatient clinics in El Azazi Hospital for Mental Health in the Al-Sharkia Governorate. Subjects: Fifty mothers of children with ADHD were specially selected as a sample for this study. Tools of data collection: The data collection sheet is composed of four parts including a socio-demographic sheet, The Zung Self-Rating Depression Scale (SDS), the Zung Self-Rating Anxiety Scale (SAS), and the Family Assessment Device - General Functioning Scale. Results: Concerning levels of depression, anxiety and family functioning among mothers of children with ADHD, two-fifths of them had a mild to moderate anxiety score. Also, half of mothers had a mild depression score, and about three-quarters had problematic general family functioning. Conclusion: Significant positive correlation was observed between depression, anxiety, and family functioning among mothers of children with ADHD. Recommendations: Provide evidence-based parent training programs focusing on managing ADHD-related behaviors and improving parenting skills. These programs can reduce anxiety and depression, and improve family functioning.