Due to the prevalence of FF, its complexes with (CoII, NiII, and CuII) were prepared, characterized, and the effect of these metal ions on the biological effectiveness of FF was studied. Elemental analysis; IR and 1H-NMR were used to determine the structures and mode of bonding, TGA, and DSC techniques were used to make thermal analysis for the prepared complexes. TGA was used to study the mechanisms of the thermal decomposition for florfenicol complexes. The prepared complexes are thermally stable and started to decompose after 200οC. Broido's method was used to determine the
activation energy for the prepared compounds, while thermal transitions and kinetic studies were carried out by differential scanning calorimetry technique (DSC). The antimicrobial activity of the complexes of florfenicol with different concentrations (0.005, 0.01, 0.02 g/mL) was examined by the agar prolixity technique against common human
pathogenic Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative). Comparatively, Nickel and Copper complexes showed a higher zone of inhibition against the tested pathogens. The antibiotic complexes were more active than the ligand itself for some breeds.