The present study aims to investigate some heavy metal concentrations in water and fish organs (liver, gills, muscles) of O. niloticus. Water samples were taken from the two different farms by a PVC tube column sampler at depth of half meter from the water surface during the period from December 2018 to May 2019 taken to lab. of central lab for Aquaculture Research (El-Abbasssa), Sharkia, Samples were collected from two different fish farms, Abbassa fish farms that located in Abbassa District, Central Laboratory for Aquaculture Research (CLAR) Abou Hammad, Sharkia, and the second sampling area is private fish farm in the way of Ismailia canal, Sharkia Governorate. In the 1^st fish farm the mean metals concentration in the water of 1^st fish farm decreased in the order: Fe > Mn > Zn >Cu > Cd > Pb, while in the 2^ndfish farm was Fe > Mn > Zn > Cd > Cu> Pb. All mean metals (Zn, Mn, Cu and Pb) concentrations in water were under the guiding lines set by (ECS, 1994). While Fe and Cd concentration their ratio exceeded the guiding lines. Fe concentration was 2.13 mg/L and 5.93 mg/L in both 1^st and 2^nd fish farm respectively and Cd concentration was 0.02 mg/L and 0.05 mg/L in both 1^st and 2^nd fish farm respectively. While Fe exceeded the guiding line value by 1.13 mg/L and 4.93 mg/L in both 1^st and 2^nd fish farm respectively, Cd also exceeded the guiding line value by 0.01 mg/L and 0.04 mg/L in both 1^st and 2^nd fish farm respectively.The mean metals concentrations in the fish muscle tissues exceed permissible limits in the 1^st fish farm was (Cu) exceeded by 0.9 mg/g and exceeded by1.69 mg/g in 2nd fish farm and (Fe) exceeded only by11.96 mg/g in the 2^nd fish farm.