A 56 days feeding trial was conducted to investigate the optimum dietary protein and stocking density that could give optimum growth performance of Pangasius catfish, Pangasius hypophthalmus. 24 tanks (150 Liter/ in each pond) were used in this study. P. hypophthalmus fish were exposed to 9 treatments; (trireplicates / each). Three experimental diets were formulated from a combination of three protein levels (30, 35 and 40%) three stocking density levels (10, 15 and 20 fish/150 L) were formulated. Pangasius catfish fingerlings (5.5 g) were fed one of the tested diets up to apparent satiety twice a day at 09:00 and 14:00 h. The diet containing 35/10 protein/ stocking density could be deemed suitable for optimal fish growth and survival. Results show that, the optimum feed utilization parameters were also recognized in fish fed the above diets. No significant differences were recorded in lipid and ash contents in the body of fish fed diets containing different protein and stocking density levels. Whereas, moisture and body protein content was positively affected by dietary protein levels. The results indicate that fish fed diet containing 35% protein with stocking density levels 10 fish/ 150L would be suitable for the optimum growth of Pangasius catfish, P. hypophthalmus fingerlings.