Since the start of the current century, the incorporation of enzymes that degrade phytate in chicken diets has greatly enhanced the sustainability of chicken meat production. The current study aims to ascertain the optimal effects of phytase supplementation on complete blood count (CBC), liver function tests, Ca, and P, carcass characteristics, and chemical meat analysis of Hubbard broiler chickens. A total of 270 one-day-old Hubbard broilers were distributed randomly among six groups. The experimental groups were Group 1 (G1) served as the control and was provided with standard basal diets. G2, G3, G4, G5, and G6 were supplemented with standard basal diets containing 50, 75, 100, 150, and 200g/ton of phytase, respectively. On the 35th day, five birds from every group were selected and slaughtered. The results revealed that elevating inclusion of phytase up to 150 and 200g/ton significantly increased carcass yield, protein% of breast meat and decreased abdominal fat compared to the 50, 75 and 100g/ton. CBC and serum biochemical parameters were not affected by phytase supplementation. In conclusion, incorporating phytase at higher doses in broiler diets can improve carcass yield, improve protein% of breast meat, and have no adverse effect on CBC or liver function tests.