Several disease outbreaks were observed in duck farms in Egypt leading tonervous
manifestations and high mortality.In this investigation,suspected Duck Viral Hepatitis
samples (n=38) were collected from 38commercial flocks from five Egyptian governorates
including(Sharkia, Qalyubia,Manufia,Ismalia and Dakahlia) from December 2019 to
May2022 of age ranged from 3-16 days.Theinfected flocks were suffered from nervous
signs with high mortality ranged from (48%-91%).There is no history of previous vaccination
againstDuck Hepatitis A Virus(DHAV). Clinical samples were examined by Reverse
transcription-PCRdepend on molecular identification of UTR andVP1 generespectively. The
overall positiverate was 76.3% (n = 29/38). Out of 29 positive samples, five (17,24%) are
subjected to sequencing and phylogenetic analysis . Our findings demonstrated that these
novel DHAV strains were more similar and genetically correlated to the newly reported
DHAV genotype 3 in Egypt. There were amino acid substitutions detected in VP1 gene in
comparison to the first Egyptian genotype 3 (Avihepatovirus A isolate 26 VP).