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323310

Ameliorating Effects of Kaempferol on Buffalo Oocytes Developmental Competence (Part- I)

Article

Last updated: 04 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Theriogenology

Abstract

To our knowledge, the cytoprotective roles of Kaempferol on the in vitro matured buffalo oocytes and embryos are still unknown. The current research was conducted to investigate the ideal dose of kaempferol that could be used safely in the vitro embryo production technology of buffalo as well as investigates the kaempferol effects on buffalos, cumulus cell expansion, in vitro maturation, penetration, fertilization, cleavage, morula, and blastocyst rates. Tissue culture media-199 supplemented with Kaempferol at 0, 5, 10, and 15 µg/ml was used to mature cumulus-oocyte complexes (COCs). The current study revealed that kaempferol supplementation at 10 µg/ml in the in vitro maturation medium of buffalo oocytes significantly improved cumulus cell expansion (75.33±1.75%), in vitro maturation (87.08±1.16%), penetration (78.40±3.75%), fertilization (42.80±1.15%), cleavage (28.20±3.51%), morula (21.70±2.65%), and blastocyst rates (19.90±2.45%) of buffalos. In conclusion, the presence of kaempferol in buffalo oocytes maturation medium is potentially important to regulate its developmental competence. Moreover, 10µg/ml of Kaempferol is an ideal promising dose that could be used safely to augment the in vitro embryo production technology of buffalo.

DOI

10.21608/bvmj.2023.221684.1677

Keywords

kaempferol, Buffalo, oocyte, developmental, Competence

Authors

First Name

Hagar

Last Name

Bahgat

MiddleName

S.

Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, P.O. 13736, Tokh, Kaliobia, Egypt.

Email

mohamedelraey@rocketmail.com

City

-

Orcid

-

First Name

Alaa

Last Name

Abdel-Ghafar

MiddleName

-

Affiliation

Theriogenology department -benha university- faculty of veterinary medicine.

Email

abdel_ghafar@yahoo.com

City

El- Qalibua

Orcid

-

First Name

Magdy

Last Name

Badr

MiddleName

Ramadan

Affiliation

Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute, Agriculture Research Center, Al Haram, P. O. 12556, Giza, Egypt.

Email

elraey1980@google.com

City

Giza

Orcid

-

First Name

Basant

Last Name

shafik

MiddleName

-

Affiliation

Animal Wealth Development Department, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt.

Email

basant.shafik@fvtm.buredu.eg

City

-

Orcid

-

First Name

Rasha

Last Name

Azab

MiddleName

El-sayed

Affiliation

Department of Physiology, Faculty of Veterinary Medicine, Benha University, P.O. 13736, Tokh, Kaliobia, Egypt.

Email

elraay_310@yahoo.om

City

-

Orcid

-

First Name

Sherif

Last Name

Ramadan

MiddleName

-

Affiliation

Animal Wealth Development Department, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt.

Email

sheriferamadan@fvtm.bu.edu.eg

City

-

Orcid

-

First Name

Mohamed

Last Name

Mohamed

MiddleName

El-Raey

Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, P.O. 13736, Tokh, Kaliobia, Egypt.

Email

elraay_310@yahoo.com

City

Moshtohor-Tokh

Orcid

0000-0002-1900-0065

Volume

45

Article Issue

1

Related Issue

44089

Issue Date

2023-10-01

Receive Date

2023-07-10

Publish Date

2023-10-01

Page Start

60

Page End

63

Print ISSN

1110-6581

Online ISSN

2974-4806

Article File

BVMJ_Volume 45_Issue 1_Pages 60-63.pdf

PDF . 890.1kB

Link

https://bvmj.journals.ekb.eg/article_323310.html

Detail API

https://bvmj.journals.ekb.eg/service?article_code=323310

Order

323,310

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

Ameliorating Effects of Kaempferol on Buffalo Oocytes Developmental Competence (Part- I)

Details

Type

Article

Created At

24 Dec 2024