Staphylococcus aureus is considered one of the Zooanthroponotic diseases that normally colonize skin and mucus membrane of human and can also spread from human to animals by direct or indirect contact during milking and causes disease to animal and human. The occurrence of S.aureus was determined using a conventional cultural techniques and biochemical identification. A total of 304 samples were collected from different dairy farms, 237 animal samples collected from(66 cows and 13 buffaloes).From each animal, we collected 3 samples including milk sample, teat swab and nasal swab.in addition to 15 samples were collected from dairy utensils(Hand and machine milking). As well as 52 samples from dairy workers (26 nasal swabs and 26 hand swabs).The results of nasal swabs were 3(4.55%) and 2(15.38%) from cattle and buffaloes respectively and teat swabs were 4(6.06%) and 2(15.38%) from cattle and buffaloes respectively. Also, 5(7.58%) of cow's milk were positive for S.aureus. Moreover, the results of machine and hand milking dairy utensils were 11.11% and 50% respectively. The results of dairy worker's hand and nasal swabs were 23.08% and 15.38%. S. aureus isolates revealed developed resistance to amoxicillin/clavulanic acid (96.7%), ampicillin (90%), and cefotaxime (53.3%).and greater sensitivity to Levofloxacin (100%), Ciprofloxacin (70%), Erythromycin and Azithromycin (both 56.7%), Ceftriaxone (26.7%). The risk of contamination of the milk increased by contaminated hand workers, dirty utensils with poor hygiene of the farm.