Forty-Two albino rats were allocated into six groups, each group of seven rats. Group 1 of control rats administrated saline, Group 2 was given citric acid, Group 3 received 500mg/kg b.wt of green synthesized iron oxide nanoparticles, Group 4 rats were given 15mg/kg of lead acetate daily, Group 5 were given 250 mg/kg of green synthesized mediated iron oxide nanoparticles and 15mg/kg b.wt of lead acetate day after day and Group 6 which received 500mg/kg of green mediated synthesized iron oxide nanoparticles and 15mg/kg b.wt of lead acetate day after day for 45 days. The result showed significant adverse effects of lead acetate include significant increases in serum Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), alkaline phosphatase (ALP), urea, creatinine, and uric acid. Also, it showed significant decreases in total protein and albumin. Moreover it showed increasing in Malondialdehyde (MDA) levels; whereas superoxide dismutase (SOD) and glutathione reduced (GSH) levels are declines in both hepatic and renal tissue. These results confirmed by alteration on histological architecture of both liver and kidneys. The majority of the changes in the studied parameters were reversed in groups taken green mediated synthesized iron oxide nanoparticles using Punica granatum. In conclusion, green mediated synthesized IONPs using  Punica granatum were effective in ameliorating lead induced hepato-renal toxicity in rats.