H9N2 Avian Influenza viruses have become globally prevalent in poultry, posing a genuine threat to the poultry industry and humans due to zoonotic infection and a potential pandemic. Because the H9N2 avian influenza virus subtype is known to cause respiratory diseases in a variety of avian species, the emergence of novel, highly pathogenic influenza viruses is possible. The purpose of the study was to look into the prevalence of avian influenza H9N2 in free-range ducks in Moshtohor village, Qalubia, Egypt. Blood samples were gathered from 50 free-range ducks from 13 households, and serum samples were tested for H9N2 antibodies using the hemagglutination inhibition assay (HI). The study discovered 76.9% seroprevalence of AI H9N2 in the tested ducks, indicating H9N2 infection. The findings emphasize the significance of surveillance and control measures in preventing the spread of avian influenza viruses, which can cause pandemics in humans as well as severe economic losses in poultry production.