Native chickens ascaridiosis (Gallus domesticus) is a disease caused by obligatory 
intestinal Ascaridia galli (Family: Ascarididae) that causes more economic losses in the 
chicken industry. The current study was conducted in Menouf district, Menoufia, Egypt 
from January to December 2022 to determine chicken ascaridiosis's prevalence and 
identify the recovered Ascaridia galli. Intestinal samples of 898 randomly selected from 
Baladi chickens were subjected to parasitological examination. The results revealed that 
Ascaridia galli worms were detected in 12.5 % (112 out of 898). The prevalence was 
significantly influenced by the area of the examined chickens but not by the season. 
Morphological description and molecular characterization of recovered Ascaridia galli 
were reported in this study to discriminate Ascaridia galli in infected chickens. The PCR 
of Cox 1 of Ascaridia galli produced specific bands of 533 bp. The sequence has an 
identity percentage of up to 100% with A. galli from Brazil, Denmark, and USA. The 
UPGMA phylogenetic tree showed that the sequence of A. galli Cox-1 from Egypt 
clustered with A. galli sequences from Brazil, Denmark, and Ghana. This study provides 
data on the prevalence and molecular characterization of A.galli from the Menouf district, 
Menoufia, Egypt.