Due to the considerable expense associated with cellulases and their extensive range of 
applications, numerous researchers and enterprises have been compelled to explore 
alternate avenues of production. These alternatives encompass the identification of novel 
sources and the development of innovative fermentation techniques, with the aim of 
discovering specific enzymes that exhibit enhanced stability and efficiency. Plackett-Burman 
design is primarily employed as a statistical method for the purpose of screening and 
selecting the most pertinent variables that contribute to the improvement of output. The 
study showed information on the optimal levels of each variable, their interactions with 
other variables, and their impact on product yield. This resulted in a reduction in the number 
of experiments required to optimize production for several parameters, as determined using 
statistical analysis. The results obtained from the submerged fermentation experiment 
indicated that the maximal values of cellulose activity, glucose release, and cellulose efficacy 
were 92.24, 84.9, and 31.41U/ml, respectively. In contrast, solid-state fermentation yielded 
maximum values of cellulose activity, glucose release, and cellulose efficacy at 106.36, 97.87, 
and 36.21, respectively. The study identified nine significant parameters that influenced 
cellulose production by Aspergillus niger NRLL3122. These factors included inoculum size, 
substrate concentration, incubation temperature, pH, shaking conditions, incubation time, 
peptone concentration, phosphate concentration, and urea concentration. Optimization of 
cellulase production was conducted using MINITAB 18.0 software, employing response 
optimization techniques to enhance the design properties. The experiment was conducted 
using the specified variables outlined in the Plackett-Burman design (PBD). The resulting 
enzyme activity reached 106.36 U ml -1which closely approximated the anticipated value. 
Highest level of enzyme activity was achieved using a 5% inoculum size, a substrate 
concentration of 9.6%, an incubation temperature of 28 °C, a pH of 6, an incubation time of 
96 hours, and a peptone concentration of 0.75 g/L.