The current study intended to evaluate the involvement of ticks and mosquitos in
Babesia and Theileria transmission. A total of 520 ticks and 280 mosquitoes were
collected and pooled (80 pools) from 6 localities in Menofia between May 2019
and May 2021. Ticks and mosquitoes pools were molecularly examined using
universal GF2/GR2 Babesia/Theileria primers,PCR revealed thatout of 80 pools19
(23.7%) were positive for piroplasms.One positive pool was subjected to
sequencing,the results of the sequence analysis revealed Babesia bovis. Risk factor
analysis revealed that animal-keeping togethered has a significant effect
onpiroplasms transmission, on the other hand breed, sex, age of animals, season
and location have no significant effect on piroplasms transmission. The
prevalence of piroplasms infection in animal-keeping togethered was (15time)
higher than individual animals, higher in summer season (25.7%) than winter
season (10%), higher in animals >3 year (26.3%) compared to the other age
groups, in female was (1.6 time) higher than in male, and higher (1.4 times) in
imported breeds than native breeds.