 Introduction: transplantation of mesenchymal stem cells (MSCs) provides an effective strategy to protect pancreatic tissue injury in diabetes type 1. Many factors act as obstacles to their best regenerative capacity. Aim of the work: To evaluate the role of AD-MSCs in the treatment of type 1 DM and to compare their efficacy alone and with a combination with resveratrol and long-acting insulin in the pancreas of albino rats with streptozotocin induced T1DM. Methods: 10 out of the 50 healthy adult male albino rats served as donors for stem cells and the other 40 rats were subdivided into two main groups: Group I (control group (16 rat)). Group II (STZ-diabetic group (24) rats injected with a single intraperitoneal injection of Streptozotocin 50 mg/kg BW. This group was subdivided into 4 groups. GII a: untreated rats. GII b: received 1x10⁶ of cultured rat ADMSCs once intravenously into the tail vein, GII c was given MSCs+ resveratrol in combination. GII d was given MSCs+ resveratrol+ insulin glargine. pancreatic samples were prepared for Immune histochemical study, light and electron microscopic examination, and morphometric studies. Results: The AD-MSCs-treated group revealed moderate improvement in histological structure and FBG. AD-MSCs+ resveratrol+ insulin-treated group demonstrated higher improvement than that of the MSCs treated group alone or dual combination of AD-MSCs +resveratrol. Conclusion: Better synergistically protective effect on damaged pancreatic tissues can be observed in STZ induced DM rats treated with A-D MSC coupling with resveratrol and insulin treatment