Background: Ovarian rejuvenation is a potential therapeutic approach aimed at enhancing ovarian function and improving fertility results in women with poor ovarian reserve (POR). Aim: To compare PRP and normal saline injections impacts on ovarian hormones in POR patients. Methods: This prospective interventional multi-center study included 20 patients with poor ovarian response (POR). Patients underwent laparoscopy, with Group A receiving an autologous PRP injection and Group B receiving a normal saline injection. Hormone levels, including FSH, LH, estradiol, and AMH, were monitored before and after monthly PRP applications for a duration of 6 months. Antral follicles number was also assessed prior and following PRP treatment. Results: According to the ovulation status of Group A after PRP injection, out of the 10 participants, 3 (30%) ovulated, while 7 (70%) did not ovulate. Anterior follicular count post treatment showed a significant negative correlation with FSH (p < 0.001) and LH (p=0.002). Anterior follicular count post treatment showed a significant positive correlation with E2 (p=0.001) while no significant correlation with age and AMH. Conclusions: PRP therapy leads to a substantial decrease in LH and FSH concentrations. Additionally, PRP treatment leads to a substantial elevation in estradiol (E2) levels. Antral follicle count (AFC), a marker of ovarian reserve and follicular growth, significantly increases after PRP therapy, suggesting enhanced follicular development. However, the effects on AMH levels are transient, returning to baseline by the sixth month.