Background: Prolonged cord clamping allows more time to transmission of fetal blood in the placenta to the baby during birth. The study aimed to define the influence of neonatal cord clamping time on first breath, Oxygen saturation and heart rate throughout neonatal resuscitation as well as its relationship to clinical outcome. Methods: This study included 130 neonates, divided in to 2 groups: Group I were subjected to delayed cord clamping after 2 minutes (the intervention group). Group II were subjected to immediate cord clamping (the control group). Results: Both groups differed significantly in the oxygen saturation (p < 0.001), with group I higher values of 1st minute (89.51 ± 2.23 vs. 85.15 ± 3.33), 5th minute (93.75 ± 2.3 vs. 91.55 ± 2.78), and 10th minute (96.71 ± 2.22 vs. 95.03 ± 3.04) saturation, Both groups differed significantly in the heart rate (p < 0.001), with group I lower values of 1st minute (118.29 ± 8.93 bpm vs. 132.88 ± 9.63 bpm), 5th minute (133.29 ± 6.81 bpm vs. 146.6 ± 9.33 bpm), the 10th minute (151.06 ± 8.66 bpm vs. 140.72 ± 7.6 bpm) heart rate. In respect of Apgar score or NICU admission, there wasn't any statistically significant difference in either of the 2 groups. Conclusion: In the first, fifth, and tenth minutes, DCC had highest oxygen saturation and lower heart rates.