Background: COVID-19 is an international health emergency of global concern. Physicians and nurses are more susceptible to COVID-19 infection. Therefore, doctors and nurses on the front lines must have essential COVID-19 awareness, knowledge and preparedness. Objectives: To assess awareness, practices and preparedness level among healthcare workers at BUH to fight against Covid-19 and to inspect environmental readiness. Methods: This was a cross-sectional study. Data were gathered using questionnaire which were filled out by physicians and nurses. The questionnaire included questions about respondents' demographics and 39 questions concerning knowledge, practices, preparedness and barriers. Overall, 175 physician and 150 nurse had participated in the study. And observational environmental checklist including 49 questions. Results: About 80.6% of physicians and 71.3% of nurses show satisfactory score of knowledge about Covid-19. About 70.9% of physicians had adequate score of practices while near half of nurses (46%) had inadequate score of practices towards COVID-19. About 73.1% of physicians and 60% of nurses had adequate preparedness. There was a significant variation between score of practices and preparedness of physicians and nurses(p < 0.05). Overcrowding in emergency room and limitation of infection control material were the most perceived barriers towards control of COVID-19. Inspection of environmental aspects of isolation unit revealed that cleaning, disinfection and waste disposal had the highest percentage of maximum score (100%), while communication had the least percentage of maximum score (20%). Conclusion: There was discrepancy between physicians and nurses regarding practice and preparedness. Communication had the least percentage of maximum score of environmental aspect.