Introduction: Irritable bowel syndrome is a worldwide functional gastrointestinal illness, but its main cause is not definite. The primary cause of gastritis, peptic ulcers, and stomach cancer is Helicobacter pylori. The exact relation between H. pylori infection and IBS incidence is still indefinite.

Aim of the work: To assess any potential link between Helicobacter pylori infection and the incidence of irritable bowel syndrome.

Patients and method: In our study, we recruited 95 patients. They were divided into Group (1) including those who fulfilled the IBS diagnosis (in compliance with Rome IV criteria) named as a case group and a healthy control group with no IBS diagnosis as Group (2).

Every patient enrolled in the study completed a Questionnaire, completed physical examination, H. pylori stool antigen, and stool examination. Results: IBS patients were significantly (P value= 0.001) younger than the control group (32.35± 13.003 vs 41.60± 12.535). Most IBS patients were females (60%) but with no significant difference over males. H. pylori-positive patients were significantly (P value= 0.001) older than the control group (40.47± 12.572 vs 30 ± 13.149). However, there was no discernible gender or stress difference between the two groups. Age (P=0.000) and stress (P=0.000) were discovered through multivariate analysis to be independent predictors of IBS, although H. Pylori infection was not.

In conclusion, there was no connection between H. pylori infection and IBS.