The present study was conducted on a fertile eggs of total number 200 of broiler Ross 308 from 31weeks laying flock, their weight ranged from (49.2-58.9gm). The eggs were inserted to an automatic incubator according tostandard. procedures (37.5 of temperature and 60% of RH). Eggs were candled and un fertilized eggs were removed at day 6 of incubation. At the same day of injection, the remaining were randomly subdivided into 3groups at incubator each set contain 60 egg. Solutions for injection were freshly prepared at the same day of injection; ascorbic acid was dissolved atphysiological saline 0.9 % then fertilized with 0.22Mm filter paper and subsequently placed in incubator 2 hours before injectional isotonic saline (0.9%), and Ascorbic acid injected group (3 mg of pure ascorbic acid with 0.6ml of physiological saline). Histological examination of Ascorbic acid group showed highlighting typical structure of hepatic tissue with intact central vein endothelial lining, highlighting obvious regular organization of muscle fibers with multinucleated light vesicular & elongated peripheral nuclei, highlighting obvious improvement along intestinal tissue structure with increasing in intestinal villi length with significant difference than control negative group as well as control positive group. Hatchability percentage noted better in ascorbic group than other groups. Also, platelet count, White blood cells count, monocyte level was better in ascorbic group than other groups. Also, platelet count, White blood cells count, monocyte level was better in ascorbic group.