Introduction: Nanotechnology is becoming of increasing interest in medicine. Nanoparticles have a large surface area-to-volume ratio which increases their interaction with the tissues and offers improved penetration into wounds. Aim: This research aimed to evaluate and compare the effect of silver nitrate, zinc oxide, Nano silver and Nano zinc oxide on promoting healing of induced traumatic ulcer in the buccal mucosa of Albino rats. Materials and methods: Sixty adult male albino rats were divided randomly into six groups, 10 rats for each group: Group I: was a normal group. Group II: left without treatment. Group III: twice daily topical application of silver nitrate solution. Group IV: twice daily topical application of nano silver solution. Group V: twice daily topical application of zinc oxide solution. Group VI: twice daily topical application of nano zinc solution. Each group except Group I divided into two subgroups: subgroup 1 sacrificed after 7 days and subgroup 2 sacrificed after 14 days. Results: Histological sections of buccal mucosa of rats showed after 7 days loss of epithelium, lamina propria showed degeneration in collagen fibers, inflammatory cells infiltrations and dilation in blood vessels with engorgement of blood inside them. After 14 days there was improvement in healing. While nanomaterials showed the best wound healing. Conclusions: Infections may delay and deteriorate wound healing, so antimicrobial agents should be used. Nano silver improve healing faster than silver nitrate, zinc oxide and nano zinc