Introduction: The morphological differences between buccally and palatally impacted maxillary canines and their adjacent lateral incisors have always been thought to be an indicating factor for the impaction site. Aim: The present study was conducted to compare the morphological differences of maxillary canine and adjacent lateral incisor in buccally versus palatally impacted canine cases using Cone Beam Computed Tomography (CBCT). Material and Methods: Forty -two CBCT scans of patients having unilateral impacted maxillary canine were collected from the archives of the Oral Radiology department, Faculty of Dentistry, Suez Canal University. The scans were divided into two buccal and palatal impaction study groups, each comprising 21 scans. The contra-lateral normally erupted canine side was used as control for comparison with each study group. The following parameters were evaluated using On Demand3DApp software for both the canine and the adjacent lateral incisor in the study and control groups: Mesiodistal width of the crown, Anatomic height of the crown, Length of the root, Volume of the crown, Volume of the root and density of the bone surrounding the maxillary canine. Results: Buccally impacted canines and their adjacent lateral incisors showed increase in morphological parameters compared to their control side. Palatally impacted canines showed no statistically significant differences compared to the control side, while their adjacent lateral incisor showed a decrease in size compared to the control side. Conclusion: Buccally impacted canines and the adjacent lateral incisors are larger in size compared to normal erupted corresponding canines in unilateral impacted canine cases. Palatally impacted canines are of equal size as their unimpacted canines, while their adjacent lateral incisors are smaller in size than their counterparts