The current study aimed to evaluate the physiological and clinical anesthetic effects of perioperative single oral dose of pregabalin prior induction of anesthesia with ketamine in male dogs and cats. Twenty-four animals (12 dogs and 12 cats) were examined. No other preanesthetic sedative or analgesic were used to avoid masking. Animals were randomly and blindly divided into two groups. Ketamine only group and pregabalin/ ketamine group. Behavioral, anesthetic, and analgesic effects were estimated post administration of pregabalin. Onset, duration, and recovery from anesthesia were compared between the animal related groups. Results showed that pregabalin produces relevant perioperative sedation and analgesia in dogs and cats which was more relevant in cats. Good muscle relaxation in both animals was also noted. The onset of anesthesia was not significantly (≤ 0.05) different between the groups. Duration of anesthesia was prolonged in the pregabalin/ ketamine groups. Recovery was smooth and prolonged than ketamine only group in dogs and cats. Physiological parameters were not significantly (≤ 0.05) different in all groups than baseline measures. In conclusion, a single perioperative oral dose of pregabalin at 5 mg/kg is effective to produce preanesthetic sedation, preemptive analgesia and prolong induction time produced by intravenous ketamine in dogs and cats without significant physiological side effects.