Brucellosis is considered one of the highly contagious disease in animals and is zoonotic disease for human causing undulant fever. So, the objective of this study is to detect the seroprevalence of brucella among camel and detect the brucella species by PCR. A total of 200 serum samples were collected from camel in Aswan governorate and were subjected for mRBPT, CFT. The seropositive sample of mRBPT and some negative serum samples were subjected for PCR to detect Brucella species. the obtained results in this study show that 6% of serum samples were positive by mRBPT. while only 4 % of serum samples were positive by CFT. 9 out of 12 mRBPT positive serum sample were positive by PCR and the Brucella meltiness were detected in 6 out of 9 serum samples and the Brucella suis were detected in 3 serum samples out of 9 positive PCR serum samples.in conclusion detection of brucellosis in camel causing highly economic losses and causing public health hazards on human being