Spirulina platensis is an efficient feed supplement used to improve animal health and growth. Transportation of calves over long distances leads to oxidative stress. The present study was conducted for the determination of biomarkers of transport stress on calves and the evaluation of Vitamin E (Vit E), Selenium (Se), and Spirulina platensis powder in the ration of calves on oxidative stress biomarkers, liver and kidney function tests, glucose, insulin, and haptoglobin levels. Currently, a total of 40 female calves imported from Brazil, aged about 8 months, were used for this study. The samples were collected once at the quarantine facility and then three times on the farm. According to the findings, there was a significant increase in ALT, AST, MDA, haptglobin, urea, and glucose, as well as a significant decrease in GSH, SOD, CAT, and TAC levels in the stressed group when compared to the control group; a significant decrease in ALT, AST, MDA, haptglobin, and glucose levels in the Spirulina platensis and Vit E and Se groups when compared to the control group There was a significant increment in insulin level in the Vit. E and Se group when compared to the control group.