From Mar. 2020 to Apr., 2022, a total of 350 imported African breeds were subsequently clinically inspected for signs of respiratory troubles in Abu-Simbel Quarantine Station. One hundred twenty (34.28%) of the inspected cases showed remarkable signs of Bovine Respiratory Disease. Seventy-five cases of the inspected cases underwent emergency necropsies, and their lungs experienced culture testing for Mycoplasma infection. Grossly, the lungs demonstrated several regions of characteristic sequestrations in the investigated cases (66/75, 88%) with significant thickening and fibrosis of the interlobular septa. Furthermore, the tiny airways were clogged with caseated purulent exudate and had caseous necrosis bronchopneumonia. The culturally tested lungs were Mycoplasma were positive. According to the genus determination by digitonin test the eighty isolated strains were Mycoplasma. Mycoplasma bovis was molecularly positive using PCR with species-specific primers. The imported African breeds cattle may plays a crucial role in the spread of Mycoplasma illness. The outcomes are extremely indicative. A vaccination program against Mycoplasma bovis is obligatory as it is a prominent pneumogenic agent of BRD.