The transition period is defined as the period from three week before calving until three week after calving, however there is insufficient available data reflecting changes in this period in buffaloes. The present study is carried out in Assuit Governorate, Egypt on 30 Egyptian buffalo cows from 4 to 6 years old .The average body weight between 350 and, 450 kg and her average daily milk yield was 10 kg per animal/day during the transition period. Whole blood samples were collected from these buffaloes for determination of Liver function enzymes (Aspartate aminotransferase, Alanine aminotransferase, lactate dehydrogenase, Total bilirubin and Total proteins), Kidney function test (Blood urea nitrogen and Creatinine), some Lipid profile (Total cholesterol and Triglyceride) and Minerals (Calcium, Potassium and Chloride). Blood samples were collected seven times from every animal every week three times before, after and during parturition. The result appeared that a highly significant increase in Aspartate aminotransferase, Alanine aminotransferase, Creatinine, Total cholesterol, Triglyceride and calcium in the Pre-partum period. But Lactate dehydrogenase, Total bilirubin, Total protein and, Blood urea nitrogen were a highly significant increase in Post-partum period. Non-significant changes in Potassium and Chloride along transition period.