This investigation was conducted at the plant tissue culture laboratory of the plant production department, Faculty of Agriculture Saba-Basha, Alexandria University, Egypt, during the period from 2019 to 2023. This study was conducted to establish the best technique for micropropagation of the Egyptian native cultivar of taro (Colocasia esculenta var. esculenta). The traditional method of propagation could not produce the high demand and quality planting materials due to its low productive capacity and viral disease transmission. Therefore, this study was carried out to develop a micropropagation protocol for taro using auxiliary buds. At the initiation stage, MS (Murashige and Skoog media) was supplemented with different types and concentrations of plant regulators such as benzylamino purine (BAP)), indole-acetic acid (IAA), and Naphthalene acetic acid (NAA) for initiation, shoot multiplication and root induction.  There was a significant difference among growth regulators at the initiation, multiplication and rooting stages. The highest culture initiation growth was obtained on MS medium supplemented with 0.50 mg/l of BAP and NAA. Also. the maximum number of shoots was obtained on MS + 0.50 mg/l of BAP and 0.50 mg/l of NAA. Roots and root length per plantlet were significantly recorded on MS media supplemented with 2 mg/l of IAA and 3 mg/l of NAA, respectively. Furthermore, at the acclimatization stage, the highest survival percentage was recorded at 100% and 86 % after 45 and 60 days respectively, with plants that transferred on a cultivated substrate containing peat moss+ vermiculate at a ratio (1:1). This micropropagation protocol can be used to produce quality and true-to-type taro planting material for large scale for commercial production.