This study aimed to assess the level of knowledge, attitudes, and practices among workers and milk producers towards bovine brucellosis in the Hantoob administration unit-Gezira State, Sudan in 2021. A Cross-sectional with a randomized sampling technique was used, 85 workers and milk producers from 22 villages in the Hantoob administration unit were involved in the study. A structured questionnaire was used to assess knowledge, Attitudes, and Practices among workers and milk producers. The data were collected from the workers and milk producers through the questionnaire methods. Results found that the knowledge of the workers and milk producers was poor about Brucellosis that (59%) did not know about brucellosis. The majority of those who knew about brucellosis (80%) knew about it from their friends, and (96%) didn't know about the causative agent of brucellosis. Most workers and milk producers (80%) did not know the mode of brucellosis transmission and did not realize that brucellosis is transmitted from animals to humans. Most workers and milk producers (84%) showed ignorance about the symptoms of brucellosis in animals, while (92%) did not know the symptoms in humans, and 87% of the workers and milk producers didn't know the prevention methods of brucellosis. Most of the workers and milk producers (64%) didn't isolate the aborted animals and left them with other animals. The majority of the workers and milk producers (91%) drank raw milk, and (82%) of them were non-cocked meat, this Study also shows that there was no periodic checkup or Immunization for brucellosis in animals in our area study (96%). No immunization for brucellosis in animals in our area study (96%) and Based on this study.