Banana is a very important fruit crop in Egypt after citrus, grapes, and mangos. High morphological and horticultural variations were recorded between the banana cultivars worldwide. In this study, the genetic diversity between seven common banana cultivars grown in Egypt was evaluated using inter-simple sequence repeat (ISSR) markers. Total DNA extracts were prepared and used as templates for PCR reaction in the presence of six primers. These primers generated 51 DNA fragments containing 50 polymorphic bands and one monomorphic band. The ISSR-PCR primers were varied in their amplified DNA fragments which ranged between 5 to 11 bands. The fifty DNA polymorphic fragments differentiated between the seven banana cultivars under investigation which belong to different banana groups. Similarities between these cultivars ranged from 27.57 to 80.18%, which proved the genetic variations between these cultivars and also confirmed the morphological and horticultural differences between them. The highest similarity of 80.18% was found between Williams and Baladi (Unknown) cultivars, while the lowest similarity was between Maghrabi and Baladi banana cultivars. A dendrogram of phylogenetic relationships confirmed the genetic and morphological diversity between the seven banana cultivars. The experimental results revealed the importance of ISSR markers in distinguishing between the banana cultivars