The ability of K.Pneumonia isolates to form biofilm was tested using the calibration plate method. The results showed that all isolates of which 50 (100%) have the ability to form biofilm, as 40 isolates (80%) were strong for biofilm formation, while 8 isolates (16%) were medium and 2 (4%) were weak for biofilm formation.
           The antibiotic effect of zinc nanoparticles on biofilm formation showed that out of 50 K. Pneumoniae isolates, the biofilm of 14 isolates was inhibited by (28%), and the highest inhibition rate of biofilm was at a concentration of 64 μg /ml.
           The antibiotic showed that the effect of zinc nanoparticles, ZnFe₂O₄ on the production of urease enzyme for 50 isolates of K. Pneumoniae (10 isolates) was inhibited by 20% on the production of urease enzyme, and 40 isolates by 80% were urease-producing.