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303300

Screening and Optimization of the Production of Alpha-Amylase Enzyme from Streptomycetes

Article

Last updated: 24 Dec 2024

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Tags

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Abstract

Amylases are enzymes that help starch break down into sugars. These Enzymes make up about 25% of the market for enzymes and have numerous industrial applications. Amylases are virtually entirely gone supplanted chemical methods in the starch hydrolysis business, microbial amylases being the most abundant and widest range of applications in the industries related to food, fermentation, textiles, and paper. Many different types of plants have developed this enzyme, including microorganisms like bacteria, fungi, algae, and actinomycetes. 37 isolates of the actinomycetes from various Egyptian governorates' soil were used in this investigation. Only 12 Streptomyces isolates were found to have the best capacity for the production of extracellular alpha-amylase, as shown by the existence of a clear zone surrounding their colonial growth.  Starch and yeast extract were efficient suppliers of carbon and nitrogen, respectively. Alpha amylase requires a temperature of 30 degrees, a pH of 7, and an incubation period of seven days.

DOI

10.21608/eajbsg.2023.303300

Keywords

Actinomycetes, alpha-amylase, isolation, Screening, Optimization

Authors

First Name

Hadir

Last Name

Sobhy

MiddleName

F.

Affiliation

Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt.

Email

hadirfawzy94@yahoo.com

City

Egypt

Orcid

-

First Name

Mohamed

Last Name

El awady

MiddleName

E.

Affiliation

Microbial Biotechnology Department, Biotechnology Research Institute, National Research Centre, El-Buhouth St. 33, Dokki, Cairo, Egypt.

Email

-

City

Egypt

Orcid

-

First Name

Mohamed

Last Name

El-khonezy

MiddleName

I.

Affiliation

Molecular Biology Department, National Research Centre, 33-El Bohouth St., Dokki, Giza, P.O.12622, Egypt.

Email

-

City

Egypt

Orcid

-

First Name

Mervat

Last Name

Hassan

MiddleName

G.

Affiliation

Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt.

Email

-

City

Egypt

Orcid

-

First Name

Sohier

Last Name

Abdel Salam

MiddleName

S.

Affiliation

Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt.

Email

-

City

Egypt

Orcid

-

Volume

15

Article Issue

1

Related Issue

38907

Issue Date

2023-06-01

Receive Date

2023-04-08

Publish Date

2023-06-16

Page Start

169

Page End

178

Print ISSN

2090-0872

Online ISSN

2090-0880

Link

https://eajbsg.journals.ekb.eg/article_303300.html

Detail API

https://eajbsg.journals.ekb.eg/service?article_code=303300

Order

303,300

Type

Original Article

Type Code

689

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, G. Microbiology

Publication Link

https://eajbsg.journals.ekb.eg/

MainTitle

Screening and Optimization of the Production of Alpha-Amylase Enzyme from Streptomycetes

Details

Type

Article

Created At

24 Dec 2024