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Bacterial viruses are currently used as a biocontrol agent for bacterial plant disease management. The evaluation of formulated bacteriophages mixed with carriers as a biocontrol agent showed efficacy and reduced disease symptoms (halo blight spots HBS) on the leaves of bean plants caused by Ps. syringae. It was isolated on nutrient agar. The single colonies of Ps. syringae appeared flat and produced fluorescent diffusible pigment on the king 's B medium as well as phylogenetically based on 16s RNA gene 726 bp by PCR. Two formulated bacteria viruses were applied as a biocontrol agent to control HBS disease. A complete randomized design was adapted in two experiments with three replications under greenhouse and open field conditions. The formulated bacteria viruses (skim milk and corn flour) significantly decreased the severity of the disease under greenhouse between 88.8 and 85.3 % and under open field conditions between 74.9 and 62.0 %, in comparison to pure bacteria viruses and copper oxychloride treatments to 83.3 and 76.3% in the greenhouse in addition to 74.9 and 62.0 % in open field respectively. The use of SM and CF for disease control experiments enhanced phage lifetime by up to 120 hours and phage density by Log 1.5 and 1.2 PFU/mL in the greenhouse and open field, correspondingly. The formulated bacteriophages (CF and SM) compared with bacteria viruses and copper oxychloride in a greenhouse and open fields showed the lowest HBS and AUDPC values. These phages may be effectively used to control halo blight disease.
DOI
10.21608/eajbsg.2023.303299
Keywords
Plant disease, Bacteriophages, plant bacteria pathogens and biological control
Authors
Affiliation
Department of Basic and Applied Agricultural Sciences, Higher Institute for Agric., Co-Operation, Shoubra El-Kheima, Cairo, Egypt.
Email
abeerfaisal1523@yahoo.com
MiddleName
-Affiliation
Department of Plant Pathology, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
Email
ahmed_bondok@agr.asu.edu.eg
Affiliation
Botany and Microbiology Department, Faculty of Science, Banha University, Banha 13511, Egypt.
Email
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https://eajbsg.journals.ekb.eg/article_303299.html
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https://eajbsg.journals.ekb.eg/service?article_code=303299
Publication Title
Egyptian Academic Journal of Biological Sciences, G. Microbiology
Publication Link
https://eajbsg.journals.ekb.eg/
MainTitle
Controlling Pseudomonas syringae Causing Halo Blight on Bean Plants Via Biocontrol-Formulated Bacterial Viruses