Beta
303299

Controlling Pseudomonas syringae Causing Halo Blight on Bean Plants Via Biocontrol-Formulated Bacterial Viruses

Article

Last updated: 24 Dec 2024

Subjects

-

Tags

-

Abstract

Bacterial viruses are currently used as a biocontrol agent for bacterial plant disease management. The evaluation of formulated bacteriophages mixed with carriers as a biocontrol agent showed efficacy and reduced disease symptoms (halo blight spots HBS) on the leaves of bean plants caused by Ps. syringae. It was isolated on nutrient agar. The single colonies of Ps. syringae appeared flat and produced fluorescent diffusible pigment on the king 's  B medium as well as phylogenetically based on 16s RNA gene 726 bp by PCR. Two formulated bacteria viruses were applied as a biocontrol agent to control HBS disease. A complete randomized design was adapted in two experiments with three replications under greenhouse and open field conditions. The formulated bacteria viruses (skim milk and corn flour) significantly decreased the severity of the disease under greenhouse between 88.8 and 85.3 % and under open field conditions between 74.9 and 62.0 %, in comparison to pure bacteria viruses and copper oxychloride treatments to 83.3 and 76.3% in the greenhouse in addition to 74.9 and 62.0 % in open field respectively. The use of SM and CF for disease control experiments enhanced phage lifetime by up to 120 hours and phage density by Log 1.5 and 1.2 PFU/mL in the greenhouse and open field, correspondingly. The formulated bacteriophages (CF and SM) compared with bacteria viruses and copper oxychloride in a greenhouse and open fields showed the lowest HBS and AUDPC values. These phages may be effectively used to control halo blight disease. 

DOI

10.21608/eajbsg.2023.303299

Keywords

Plant disease, Bacteriophages, plant bacteria pathogens and biological control

Authors

First Name

Abeer

Last Name

Faiesal

MiddleName

A.

Affiliation

Department of Basic and Applied Agricultural Sciences, Higher Institute for Agric., Co-Operation, Shoubra El-Kheima, Cairo, Egypt.

Email

abeerfaisal1523@yahoo.com

City

Egypt

Orcid

0000-0002-3890-3155

First Name

Ahmed

Last Name

Bondok

MiddleName

-

Affiliation

Department of Plant Pathology, Faculty of Agriculture, Ain Shams University, Cairo, Egypt

Email

ahmed_bondok@agr.asu.edu.eg

City

Egypt

Orcid

0000-0002-2375-2956

First Name

Noha

Last Name

El Dougdoug

MiddleName

K.

Affiliation

Botany and Microbiology Department, Faculty of Science, Banha University, Banha 13511, Egypt.

Email

-

City

Egypt

Orcid

-

Volume

15

Article Issue

1

Related Issue

38907

Issue Date

2023-06-01

Receive Date

2023-04-08

Publish Date

2023-06-15

Page Start

151

Page End

167

Print ISSN

2090-0872

Online ISSN

2090-0880

Link

https://eajbsg.journals.ekb.eg/article_303299.html

Detail API

https://eajbsg.journals.ekb.eg/service?article_code=303299

Order

303,299

Type

Original Article

Type Code

689

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, G. Microbiology

Publication Link

https://eajbsg.journals.ekb.eg/

MainTitle

Controlling Pseudomonas syringae Causing Halo Blight on Bean Plants Via Biocontrol-Formulated Bacterial Viruses

Details

Type

Article

Created At

24 Dec 2024