Lipases, also known as triacylglycerol hydrolases, are a class of biotechnologically essential enzymes that have several uses in the dairy, detergent, culinary, and pharmaceutical sectors. Microbes are primarily manufactured of lipases, and bacterial lipases in particular play a major role in commercial operations. The goal of this study is to screen and design optimal conditions for lipase synthesis in bacterial isolates. 25 bacterial isolates were collected at random from various habitats, including soil and contaminated soil, for this study. The isolates that could produce lipase were chosen qualitatively using a rapid plate test method. The following conditions were found to be ideal for lipase production: pH 7.0, incubation temperature of 40°C for 4 days, and carbon and nitrogen supplies of olive oil and peptone, respectively. The highest amounts of lipase synthesis were achieved under these conditions.