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289460

Screening for the Production of MetNase and Its Optimum Conditions from Streptomycete Isolate

Article

Last updated: 03 Jan 2025

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Tags

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Abstract

MetNase might be a valuable source for different medical applications. MetNase has the potential to be used against a wide range of tumour cell types as an anticancer agent. The purpose of this research is to screen and develop optimal conditions of streptomycetes isolates for MetNase production. For this study, 34 randomly selected streptomyces isolates from soil and marine water were used. The isolates that can produce MetNase were qualitatively selected using a quick plate assay method. When the pH was set to 7.0, the incubation temperature was set to 30 °C, and the carbon and nitrogen sources were glucose and peptone, respectively, the highest levels of MetNase synthesis were attained.

DOI

10.21608/eajbsg.2023.289460

Keywords

Streptomycetes, MetNase, isolation, Screening, Optimization

Authors

First Name

Mohamed

Last Name

Abdelmonem

MiddleName

O.

Affiliation

Department of Botany and Microbiology, Faculty of Science, Benha University, Benha 33516, Egypt

Email

mohamed.abdelmonem@fsc.bu.edu.eg

City

Egypt

Orcid

-

First Name

Mervat

Last Name

Hassan

MiddleName

G.

Affiliation

Department of Botany and Microbiology, Faculty of Science, Benha University, Benha 33516, Egypt

Email

-

City

Egypt

Orcid

-

First Name

Mohamed

Last Name

Elawady

MiddleName

E.

Affiliation

Department of Genetic Engineering and Biotechnology National Research Center.

Email

-

City

Egypt

Orcid

-

First Name

Manal

Last Name

Mohamed

MiddleName

M.

Affiliation

Department of Genetic Engineering and Biotechnology National Research Center.

Email

-

City

Egypt

Orcid

-

First Name

Reem

Last Name

Hilal

MiddleName

I.

Affiliation

Department of Botany and Microbiology, Faculty of Science, Benha University, Benha 33516, Egypt

Email

-

City

Egypt

Orcid

-

Volume

15

Article Issue

1

Related Issue

38907

Issue Date

2023-06-01

Receive Date

2022-12-21

Publish Date

2023-03-03

Page Start

77

Page End

86

Print ISSN

2090-0872

Online ISSN

2090-0880

Article File

EAJBSG_Volume 15_Issue 1_Pages 77-86.pdf

PDF . 655.8kB

Link

https://eajbsg.journals.ekb.eg/article_289460.html

Detail API

https://eajbsg.journals.ekb.eg/service?article_code=289460

Order

289,460

Type

Original Article

Type Code

689

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, G. Microbiology

Publication Link

https://eajbsg.journals.ekb.eg/

MainTitle

Screening for the Production of MetNase and Its Optimum Conditions from Streptomycete Isolate

Details

Type

Article

Created At

24 Dec 2024