This study aimed to biosynthesize silver nanoparticles (AgNPs) using the aqueous extract of Polyporus plorans. The properties of the synthesized AgNPs were studied by UV-Vis, FTIR, AFM and SEM. The total antioxidant capacity was also detected for the first time in the world. The results showed that the AgNPs solution revealed an absorption maximum at 420 nm by ultraviolet-visible spectrum. Fourier transform infrared spectroscopy (FTIR) results showed that the AgNPs solution contained many functional groups belonging to the carbonyl group (C = O).  The atomic force microscope (AFM) showed synthesized AgNPs were spherical in shape and the average size of the nanoparticles was 88.9 nm. The results of the scanning electron microscope (SEM) showed the prepared silver nanoparticles were in nano sizes 36.61-59.30 nm. The prepared AgNPs exhibited different antimicrobial activities against the diverse microorganisms at concentrations of 100%, 50%, and 25%. The AgNPs including pathogenic bacteria, Escherichia coli and Staphylococcus aureus, and some yeasts, Candida galbrata, C. krusei, C. tropicalis, C. albicans, and two dermatophyte species, Trichophyton rubrum and T. mentagrophyte. The results showed that the highest inhibition was for bacteria and dermatophytes at 100%, while for Candida it showed an inhibitory effect against C. galbrata and C. krusei only.The results of the antioxidant activity test showed an increase in its anti-oxidant effectiveness with an increase in the concentration solution. The results of current study indicated that the synthesized AgNPs from P. plorans may be developed as an effective agent against bacterial and fungal infections.