Aim: This study was conducted to assess the effect of preventive regimens using herbal in comparison fluoride toothpastes on remineralization of initial lesions in high risk patients. Materials and methods: Two parallel armed group, randomized trial was conducted on 32 participants with high caries risk, having initial lesions at smooth surfaces. Participants were randomly allocated into two groups, 16 participants each. Assessment of remineralization of lesions were evaluated by diagnodent, visual examination of lesions was evaluated by international caries detection and assessment scoring system. Results: Diagnodent there was no statistically difference at; baseline, 1 week, while at 3 months' intergroup comparison revealed statistically difference between both toothpastes (P=0.0155), intergroup comparison within Himalaya or Colgate have proven there is a statistically difference between the various times (P < 0.0001). ICDAs, there was no statistical difference (P = 0.1506 and P = 0.7273), intragroup comparison within Himalaya have shown statistically difference between different periods. Within Colgate, intragroup comparisons revealed no significant differences between periods. Conclusion: Preventive regimens containing different toothpastes are positively influencing remineralization of initial lesions in high risk patients, while herbal toothpaste was superior along three months, regimens didn't produce significant effect in altering visual appearance of initial lesions.