Aim:The objective of study was to evaluate the effect of cyanoacrylate tissue adhesive on healing of skin wounds versus vicryl suture among rabbits through histological examination and immunohistochemical detection of Transforming growth factor beta 1 (TGF-β1) and Tumor necrosis factor alpha (TNF-α).
Materials & Methods: This study was carried out on 10 rabbits which subjected to the same surgical procedures as skin incisions were performed. The rabbits were divided into two equal groups of five animals each, Group I (control group); Whose skin wounds were closed with 3-0 vicryl suture and Group II (study group); Cyanoacrylate Glue were applied only on the site of wound with observation periods of two weeks:
Results:The histological results showed that skin specimens of control rabbit stained with hematoxylin and eosin stain two weeks after surgery revealed formation of thin epithelial layer with absence of epithelial ridges while the skin of the experimental site revealed increased epithelial thickness. Skin wounds of control site stained with Masson's trichrome stain revealed mild positive staining reaction while skin wounds of the experimental site showed strongly positive staining reaction.The immunohistochemical localization of transforming growth factor beta (TGF-b) revealed that examination of specimens of control site showed weak positive staining reaction while skin wounds of the experimental site showed strongly positive staining reaction.Immunohistochemical localization of tumor necrosis factor alpha (TNF-α) showed that examination of skin specimens of control site showed weak to moderate positive staining reaction while skin wounds of the experimental site showed weak positive staining reaction.