Objective: To study the anti-obesity effect of oral administration of Chromax and/or Plantago ovata seed powder on female rats fed a high-fat diet. Materials and methods: The control or lean group included six females and was fed a normal fat diet for 18 weeks. While the other twenty-four were fed a high-fat diet containing 45% animal fat for 3 months to induce obesity and then randomly divided into four homogeneous groups (6 for each group) as follows: Group II or obese control group which was fed a high-fat diet throughout the experimental period. Group III or Chr which was fed a high-fat diet with 200 μg/kg/day for 12 weeks. Group IV or Pla which was fed a high-fat diet mixed with 30% Ispaghula seed powder for 12 weeks. The fifth group or Mix was fed a high fat diet with 200 μg/kg/day and 30% of ispaghula seed powder for 12 weeks. Anthropometric parameters such as (body weight change, body weight gain percentage, body mass index, and Lee index) and serum glucose were assessed along with histological examinations of fat. Results: The results showed an increase in body weight, body mass, lee indexes, abdominal fat, blood glucose, and several histopathological lesions in obese females of the second group while body weight, body mass index, and Lee index decreased and blood glucose improved in the chromax group more than the plantago seed powde. Conclusion: the mixture of chromax with plantago seed showed anti-obesity results compared to other groups.