Aim: The present study was conducted to determine the immune-enhancing effects from supplementation of Coenzyme Q10 on a cyclophosphamide-induced immunosuppressed rat model. Study Design & Setting: Quasi experimental study design was selected. The Study was placed at Animal House, Husbandry and Veterinary Sciences, Sindh Agricultural University (SAU) Tandojam and Post Graduate lab, Isra University from November 2020- April 2021.Materials & Methods: 40 adult healthy female albino Wistar Rats were selected and placed in 4 groups (n=10/group). Food and water were provided ad libitum. Control group A rats were given corn oil (100ul) intraperitoneally from Day 1-7. Experimental group B rats were given corn oil (100ul) intraperitoneally from Day 1-7 as a placebo followed by single dose of cyclophosphamide (200mg/kg) intraperitoneally on Day 8. Pre-treatment group C animals were given Co-enzyme Q10 (300mg/kg) dissolved in corn oil (100ul) intraperitoneally from Day 1- 7 followed by a single dose of cyclophosphamide (200mg/kg) intraperitoneally on Day 8. A Single dose of 200mg/kg of cyclophosphamide was initially given intraperitoneally to Post-treatment group D animals followed by Co-enzyme Q10 (300mg/kg) dissolved in corn oil (100ul) intraperitoneally from Day 2-8. On Day 9, animals were weighed and sacrificed. Spleen was preserved and prepared for light microscopy. Results: Graded white pulp atrophy was observed in animals in Groups B, C & D. Marked effectiveness of Coenzyme Q 10 was observed in animals of the pretreatment group. Conclusion: It can be concluded that Coenzyme Q10 Supplementation in the Pre-treatment Group displayed marked effectiveness in immunocompromised adult female Wistar albino rats.