Background: Extra-gastric implications of H. pylori infection have raised serious health concerns. The association of several extra-gastric diseases with H. pylori infection has been studied, in the recent past, however, information is lacking on H. pylori-associated modulation in the bone profile of the infected patients that may lead to bone disorders. The study aimed to assess HP-associated modulation in the vital components of the bone profile. Methods: A retrospective (hospital-based case-control) research (n = 385) was undertaken. H. pylori-associated alteration in the vital component of the bone profile was ascertained by Chi-square, V_Cramer, Bayesian V_Cramer, bias factor/log (BF₀₁), and logistic regression using R-packages. P < 0.05 was the cut-off for significance. Results: The difference in mean ± SD (female vs male) for uncorrected calcium (2.2 ± 0.3 vs 2.3 ± 0.3; p <0.001), albumin (37.7 ± 7.8 vs 40.7 ± 6.2; p <0.001), and alkaline phosphatase (69.3 ± 26.8 vs 77.1 ± 35.7; p = 0.016) was statistically significant. A significant difference in mean ± SD of corrected calcium of HP-infected vs HP-uninfected was measured (2.17 ± 0.3 vs 2.19 ± 0.3; p = 0.04). The proportion of hyper/hypo-calcemia, hyper/hypo-magnesemia, hyper/hypo-phosphatemia, and hyper/hypo-albuminemia was independent (no association) of HP-infection (V_Cramer ≈ 0.00, log (BF₀₁) >100, p > 0.5). The association of only hyperphosphataemia (AOR: 2.68; CI: 1.18–6.13; p < 0.05) and hyperalbuminemia (AOR: 1.25; CI: 0.47–3.35; p < 0.001) with HP-infection was significant, however, HP-infection-dependent modulation in other components of the profile was not significant (p> 0.05). Conclusion: The association of only hyperphosphatasemia and hyperalbuminemia with HP infection was significant, however, HP-infection-dependent modulation in other components of the profile was not significant. Large group well-controlled research is recommended to gain comprehensive insight into it.