A typical chemotherapy agent used to treat a number of tumors is called cisplatin. Male infertility is one of the side effects of cisplatin therapy, which is one of the drugs' side effects. Male mammalian germ cells have been shown to be protected by chlorella vulgaris supplementation. The goal of the current study was to examine, using light and electron microscopy, the protective effects of Chlorella vulgaris supplementation against damage to healthy Testis tissue caused by the cisplatin-based treatment regimen. Each of the four groups, which each had five rats, contained the animals. Saline injections intraperitoneally were given to the animals in the control group. Cisplatin family: The same dose of cisplatin utilized in the human treatment procedure, 134 mg/kg intraperitoneally, was administered to the animals for three months. For three months, animals in the chlorella group consumed 150 mg/kg of chlorella daily. Chlorella/Cisplatin group: The rats got 150 mg/kg of chlorella orally daily for three months and 134 mg/kg of cisplatin intraperitoneally once each week. Following cisplatin treatment, abnormalities in the testis were averted by chlorella supplementation, according to the results of light and transmission ultrastructure microscopy research.