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314580

Phenotypic Detection of Some Virulence Factors among Hypervirulent Klebsiella pneumoniae and Classic Klebsiella pneumoniae Associated with Urinary Tract Infections

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Last updated: 24 Dec 2024

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Abstract

The present study aimed to investigate prevalence and some virulence factors among hyper virulent Klebsiella pneumoniae (HVKP) and classic Klebsiella pneumoniae (CKP) isolated from urinary tract infections.Three hundred urine samples collected from patients with urinary tract infections were cultured on MacConkey agar for primary isolation of    K. pneumoniae isolates. Vitek 2 Compact System was used for identification of K. pneumoniae. HVKP and CKP were detected by string test.  Some virulence factors (biofilm formation, haemolysin, and urease production) possessed by the bacterial isolates were investigated phenotypically.




           Sixty bacterial isolates belonged to K. Pneumoniae where 15 (25%) isolates belonged to HVKP as viscous string measuring >5 mm in length and 45 (75%) isolates belonged to CKP where viscous string measuring <5 mm in length. The results of haemolysis test showed that 60% of HVKP isolates gave β-haemolysis, while 51.1% of CKP isolates gave β-haemolysis. All bacterial isolates (100%) of HVKP and CKP showed the ability to produce urease. Phenotypically, all HVKP and CKP isolates showed the ability to form biofilm while only 33.3% and 11.1% isolates showed high ability to form biofilm respectively by Tissue Culture Plates methods (TCP). 

DOI

10.21608/eajbsd.2023.314580

Keywords

Hypervirulent K. pneumoniae, Classic K. pneumoniae, Biofilm, haemolysin, urease

Authors

First Name

Adian

Last Name

Saudi

MiddleName

H.

Affiliation

Department of Biology, Faculty of Education for Girls, University of Kufa, Najaf, Iraq

Email

-

City

Iraq

Orcid

-

First Name

Ahlam

Last Name

Naeem

MiddleName

K.

Affiliation

Department of Biology, Faculty of Education for Girls, University of Kufa, Najaf, Iraq

Email

ahlam.alyaseen@uo.kufa.edu.iq

City

Iraq

Orcid

-

Volume

15

Article Issue

2

Related Issue

42424

Issue Date

2023-12-01

Receive Date

2023-07-25

Publish Date

2023-09-01

Page Start

27

Page End

34

Print ISSN

2090-0775

Online ISSN

2090-0848

Link

https://eajbsd.journals.ekb.eg/article_314580.html

Detail API

https://eajbsd.journals.ekb.eg/service?article_code=314580

Order

314,580

Type

Original Article

Type Code

685

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, D. Histology & Histochemistry

Publication Link

https://eajbsd.journals.ekb.eg/

MainTitle

Phenotypic Detection of Some Virulence Factors among Hypervirulent Klebsiella pneumoniae and Classic Klebsiella pneumoniae Associated with Urinary Tract Infections

Details

Type

Article

Created At

24 Dec 2024