Beta
ManageSearchChatDiscover
308360

DNA AND EDNA-BASED TECHNIQUES FOR CHARACTERIZING THREE UNIONID BIVALVE SPECIES IN THE NORTHERN RIVER NILE, EGYPT

Article

Last updated: 03 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Animal Cell Biology and Genetics

Abstract

Bivalve mollusks are avid filter feeders. They filter significant amounts of algae, bacteria, sediment, nutrients, and other particles from the waters, playing a key benthopelagic regulatory role on the freshwater ecosystems. To provide DNA-based approach and environmental DNA (eDNA) based-techniques for accurate species identification and tracking, three different unionid bivalves were collected from North of the River Nile, belonging to the genera Coelatura, Mutela, and Chambardia. They were subjected to DNA barcoding, through sequencing the hypervariable barcode region of the mitochondrial cytochrome oxidase subunit I gene (COI), and phylogenetically analyzed. Also, the resulting barcodes were used to design species-specific PCR assays that were tested over waters where the three species were co-incubated. The results revealed that the collected species were mainly Mutela rostrata and Chambardia rubens. The third species showed barcoding and phylogenetic proximity to Coelatura aegyptiaca, yet mixed identity was found with two other Northeastern African Coelatura species. eDNA-based PCRs provided specific, clear amplicons for the three species. Based on these results, it is strongly recommended to extend the application for molecular techniques for further investigation of hidden diversity in the world of River Nile bivalves in Egypt to detect their exact species, abundance, and status of conservation.

DOI

10.21608/ejz.2023.215239.1096

Keywords

<i>Chambardia</i>, <i>Coelatura</i>, DNA barcoding, Fluvial, <i>Mutela</i>

Authors

First Name

Asmaa

Last Name

Galal-Khallaf

MiddleName

-

Affiliation

Zoology Department, Faculty of Science, Menoufia University, Menoufia, Egypt

Email

as_kh_22@yahoo.com

City

-

Orcid

0000-0001-5459-843X

First Name

Asmaa

Last Name

Farahat

MiddleName

M.

Affiliation

Lakes and Fish Resources, Protection and Development Agency, Cairo, Egypt

Email

amohameda05@gmail.com

City

-

Orcid

-

First Name

Mona

Last Name

Elmadawy

MiddleName

E.

Affiliation

Zoology Department, Faculty of Science, Menoufia University, Menoufia, Egypt

Email

monaelmadawy7@gmail.com

City

-

Orcid

-

First Name

Mona

Last Name

Rezk

MiddleName

M.

Affiliation

Natural Resources Department, Faculty of African Postgraduate Studies, Cairo University, Giza, Egypt

Email

monarezk456@gmail.com

City

+201069097531

Orcid

-

First Name

Mohamed

Last Name

El-Mallah

MiddleName

F.

Affiliation

Natural Resources Department, Faculty of African Postgraduate Studies, Cairo University, Giza, Egypt

Email

elmallahmohammed9994@yahoo.com

City

-

Orcid

-

First Name

Heba

Last Name

Hathout

MiddleName

M. R.

Affiliation

Chemistry Department, Faculty of Science, Menoufia University, Menoufia, Egypt

Email

hathoutheba@cu.edu.eg

City

-

Orcid

-

First Name

Alkhateib

Last Name

Gaafar

MiddleName

Y.

Affiliation

Hydrobiology Department, Veterinary Research Institute, National Research Center, Giza, Egypt

Email

alkhateibyg@yahoo.com

City

-

Orcid

-

First Name

Khaled

Last Name

Mohammed-Geba

MiddleName

-

Affiliation

Zoology Department, Faculty of Science, Menoufia University, Menoufia, Egypt

Email

khaled-mohamed.geba@fulbrightmail.org

City

-

Orcid

0000-0002-0281-816X

Volume

81

Article Issue

81

Related Issue

47865

Issue Date

2024-06-01

Receive Date

2023-06-03

Publish Date

2024-06-01

Page Start

15

Page End

29

Print ISSN

1110-6344

Online ISSN

2682-3160

Article File

EJZ_Volume 81_Issue 81_Pages 15-29.pdf

PDF . 1.6MB

Link

https://ejz.journals.ekb.eg/article_308360.html

Detail API

https://ejz.journals.ekb.eg/service?article_code=308360

Order

26

Type

Original Research Papers

Type Code

684

Publication Type

Journal

Publication Title

Egyptian Journal of Zoology

Publication Link

https://ejz.journals.ekb.eg/

MainTitle

DNA AND EDNA-BASED TECHNIQUES FOR CHARACTERIZING THREE UNIONID BIVALVE SPECIES IN THE NORTHERN RIVER NILE, EGYPT

Details

Type

Article

Created At

24 Dec 2024