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THE ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITIES OF THE ETHANOLIC EXTRACT OF <i>TINOSPORA CORDIFOLIA</i> LEAVES: <i>IN VITRO</i> AND <i>IN VIVO</i> STUDIES

Article

Last updated: 03 Jan 2025

Subjects

-

Tags

Animal Histology
Animal Physiology

Abstract

Tinospora cordifolia has been used in Ayurvedic medicine to treat a number of diseases for a long time. The antioxidant and hepatoprotective activities of the ethanolic extract of T. cordifolia leaves (TC extract) were tested in the present study on the cultured rat hepatocytes and thioacetamide (TAA)-treated rats. For in vivo experiments, forty male Wistar rats were allotted randomly into eight groups (n=5): group “1" acted as the control group, group “2" represented the TAA-treated rats, groups “3-5" were treated with different doses of TC extract (50, 100, and 200 mg/kg body weight, respectively, orally/daily for 4 weeks); while groups “6-8" were TAA-treated rats that received 50, 100, and 200 mg, respectively, of TC extract/kg body weight (orally/daily for 4 weeks). The TC extract exhibited no marked cytotoxic activity against the cultured hepatocytes at IC50≤500 µg/mL (after 48 hours) in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The in vivo experiments revealed that the TC extract (at doses ≥100 mg/kg body weight) modulated significantly (P<0.05) the increase in the plasma aminotransferases and C-reactive protein levels, and lactate dehydrogenase activity, in the TAA-treated rats. In addition, the TC extract (at doses ≥50 mg/kg body weight) boosted significantly (P<0.05) the superoxide dismutase and catalase activities, and the glutathione content, in the hepatic tissues of the TAA-treated rats. Moreover, the histological results confirmed the hepatoprotective effects of the TC extract in the TAA-treated rats. In conclusion, the TC extract showed a hepatoprotective activity in the TAA-treated rats by its powerful antioxidant potential.

DOI

10.21608/ejz.2022.139640.1083

Keywords

acute liver injury, Cultured hepatocytes, GC-MS analysis, MTT assay, <i>Tinospora cordifolia</i>

Authors

First Name

Hanan

Last Name

Hussien

MiddleName

Tantawy

Affiliation

Zoology Department, Faculty of Sciences, Suez Canal University, Ismailia, Egypt

Email

hanantantawy226@gmail.com

City

cairo

Orcid

-

First Name

Hend

Last Name

Tag

MiddleName

M.

Affiliation

Zoology Department, Faculty of Sciences, Suez Canal University, Ismailia, Egypt

Email

hend_taha@science.suez.edu.eg

City

Ismaillia

Orcid

0000-0001-9417-1844

First Name

Eman

Last Name

Ahmed

MiddleName

-

Affiliation

Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt

Email

eman_ahmed@vet.suez.edu.eg

City

Ismaillia

Orcid

-

First Name

Zohour

Last Name

Nabil

MiddleName

I.

Affiliation

Zoology Department, Faculty of Sciences, Suez Canal University, Ismailia, Egypt

Email

zohournabil@yahoo.com

City

Ismaillia

Orcid

-

First Name

Mohamed

Last Name

El-Naggar

MiddleName

S.

Affiliation

Zoology Department, Faculty of Sciences, Suez Canal University, Ismailia, Egypt

Email

msemaenr2000@gmail.com

City

Ismaillia

Orcid

0000-0002-2546-9847

Volume

79

Article Issue

79

Related Issue

41664

Issue Date

2023-06-01

Receive Date

2022-05-19

Publish Date

2023-06-01

Page Start

48

Page End

65

Print ISSN

1110-6344

Online ISSN

2682-3160

Link

https://ejz.journals.ekb.eg/article_262157.html

Detail API

https://ejz.journals.ekb.eg/service?article_code=262157

Order

15

Type

Original Research Papers

Type Code

684

Publication Type

Journal

Publication Title

Egyptian Journal of Zoology

Publication Link

https://ejz.journals.ekb.eg/

MainTitle

THE ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITIES OF THE ETHANOLIC EXTRACT OF <i>TINOSPORA CORDIFOLIA</i> LEAVES: <i>IN VITRO</i> AND <i>IN VIVO</i> STUDIES

Details

Type

Article

Created At

24 Dec 2024