Background:  Oxidative stress, caused by an imbalance between reactive oxygen species and antioxidants in semen, can harm sperm deoxyribonucleic acid and lipids, leading to lower success rates in intracytoplasmic sperm injection. Recent research suggests that oral antioxidant supplements may alleviate this stress and improve sperm quality. However, the evidence is limited. Thus, this study aims to assess the effectiveness of these supplements in enhancing intracytoplasmic sperm injection success rates, potentially providing a solution for male.Methods: This study involved two groups of 100 infertile couples. Group 1: infertile couples due to male partner's factor. Group 2: infertile couples due to male partner's factor and obesity. All male partners received antioxidant therapy with FERTITONE X pills, taking 1 mg twice daily, once in the morning and once in the evening, for three months. Semen samples were collected from participants before and after treatment. Additionally, the outcomes of intracytoplasmic sperm injection and pregnancy rates were evaluated.Results: The major finding from this study was that antioxidant therapy led to significant improvements in fertilization (p < 0.001) and cleavage (p < 0.001) in group 2, along with a notable increase in clinical pregnancy rates (42.0% to 52.0%). Significant changes in routine sperm parameters were also observed in all groups. Conclusion:  In conclusion, antioxidant therapy with FERTITONE X pills demonstrated substantial benefits in improving fertilization and cleavage rates among infertile couples undergoing intracytoplasmic sperm injection. The observed increase in clinical pregnancy rates further supports the potential efficacy of antioxidant supplementation in enhancing reproductive outcomes in male infertility treatments. These findings underscore the importance of antioxidant management in improving sperm quality and subsequent fertility outcomes.