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371518

Evaluation and Modification of Some Traditional Methods to Extract Genomic DNA in High Quality and Quantity from Different Plant Families

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Last updated: 03 Jan 2025

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Abstract

Background: Plants are rich in complex compounds, which hinder obtaining intact and pure DNA, especially by traditional manual methods. Extraction of high-quality and quantity genomic DNA is the most important step for all DNA-based biological applications, such as polymerase chain reaction (PCR), cloning, and sequencing. In this study, four DNA extraction modified protocols (CTAB, TNES) and (CTAB, TENS with a solid-phase column) were evaluated to produce highly purifiedand high-quantity DNA from the leaves and seeds of 11 species. They were compared with a kit (QIAGEN) which was used as a positive control. The techniques were evaluated for their capacity to produce DNA devoid of sugars, polyphenols, RNA, and other significant contaminants, as well as their apparent, viscosity, OD260 reading, and appropriateness for PCR-based assays. Results: The most obvious result was that using traditional methods such as CTAB and TENS with a solid-phase column increased the efficiency of the method and gave better results in spectrophotometer readings and polymerase chain reaction. Conclusion: To get results comparable to those from Kit procedures, it is possible to enhance the conventional DNA extraction techniques. To achieve high-quality DNA at the lowest possible cost, this is accomplished by combining several extraction buffers and utilizing a solid phase column.

DOI

10.21608/eajbsc.2024.371518

Keywords

DNA extraction protocols, Solid-phase technology, molecular markers, PCR

Authors

First Name

Mona

Last Name

Moghazee

MiddleName

M.

Affiliation

Genetic Department, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shoubra 11241, Qalyubia , Egypt.

Email

m_moghazee@agr.asu.edu.eg

City

Cairo

Orcid

0000-0003-3700-3929

First Name

Shaimaa

Last Name

Abdelaziz

MiddleName

A.

Affiliation

Genetic Department, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shoubra 11241, Qalyubia , Egypt.

Email

shaimaa11@agr.asu.edu.eg

City

Cairo

Orcid

-

First Name

Rehab

Last Name

Hasanien

MiddleName

A.

Affiliation

Genetic Department, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shoubra 11241, Qalyubia , Egypt.

Email

hasanienrehab@gmail.com

City

Cairo

Orcid

-

First Name

Rawan

Last Name

Zaid

MiddleName

S.

Affiliation

Genetic Department, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shoubra 11241, Qalyubia , Egypt.

Email

rawansabryzaid@gmail.com

City

Cairo

Orcid

-

First Name

Rawan

Last Name

Fawzy

MiddleName

Y.

Affiliation

Genetic Department, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shoubra 11241, Qalyubia , Egypt.

Email

rawanyasser03@gmail.com

City

Cairo

Orcid

-

First Name

Khaled

Last Name

Mohamed

MiddleName

T.

Affiliation

Plant Department, Genetics Division, Faculty of Agriculture, Al-Azhar University, Egypt.

Email

-

City

Cairo

Orcid

-

First Name

Ahmad

Last Name

Suliman

MiddleName

A.

Affiliation

Horticultural Crop Technology Department, Agricultural and Biological Institute, National Research Centre, Dokki, Egypt.

Email

a_elsagheer2006@yahoo.com

City

-

Orcid

-

First Name

Ghada

Last Name

Samaha

MiddleName

M.

Affiliation

Field Crops Research Department, Agricultural and Biological Institute, National Research Centre, Dokki, Egypt.

Email

ghka_sam@yahoo.com

City

-

Orcid

-

First Name

Hala

Last Name

Zoghly

MiddleName

M.

Affiliation

Genetic Department, Faculty of Agriculture, Ain Shams University, P.O. Box 68, Hadayek Shoubra 11241, Qalyubia , Egypt.

Email

halazoghly@agr.asu.edu.eg

City

-

Orcid

0000-0002-2114-3700

Volume

16

Article Issue

2

Related Issue

48899

Issue Date

2024-12-01

Receive Date

2024-06-30

Publish Date

2024-08-07

Page Start

63

Page End

74

Print ISSN

2090-0767

Online ISSN

2090-083X

Article File

EAJBSC_Volume 16_Issue 2_Pages 63-74.pdf

PDF . 1.0MB

Link

https://eajbsc.journals.ekb.eg/article_371518.html

Detail API

https://eajbsc.journals.ekb.eg/service?article_code=371518

Order

371,518

Type

Original Article

Type Code

673

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

Publication Link

https://eajbsc.journals.ekb.eg/

MainTitle

Evaluation and Modification of Some Traditional Methods to Extract Genomic DNA in High Quality and Quantity from Different Plant Families

Details

Type

Article

Created At

24 Dec 2024