Molecular diagnostics is an excellent tool used by healthcare management for infectious diseases to detect and manage infections. As such, many researchers have addressed the ideas surrounding the molecular diagnosis. This review explores the role of molecular diagnostics in managing and diagnosing infectious diseases, highlighting its potential to prevent catastrophic outcomes. The literature surrounding the historical aspects and the importance of molecular diagnostics in modern healthcare can help explain the role of the tool and how it can improve the patient's outcome and help the therapeutic decisions. In this literature review, databases are searched using relevant keywords to gather information on the role of molecular diagnostics in managing and diagnosing infectious diseases. The review highlights the significance of molecular diagnostics in detecting resistant strains and guiding evidence-based antimicrobial treatment to combat antimicrobial resistance. Furthermore, the review discusses how adopting new diagnostic approaches as standard practice in clinical departments introduces various subjects. The findings demonstrate that implementing molecular diagnostics in clinical settings reduces mortality rates, improves healthcare efficiency, and enhances public surveillance.